Journal: International Journal of Molecular Sciences

Article Title: Mechanism Actions of Coniferyl Alcohol in Improving Cardiac Dysfunction in Renovascular Hypertension Studied by Experimental Verification and Network Pharmacology

doi: 10.3390/ijms251810063

Figure Lengend Snippet: Intersection targets of CA and RH.

Article Snippet: Antibodies against cyclooxygenase 2 (COX2) (sc-514489) and matrix metallopeptidase 9 (MMP9) (sc-393859) were procured from Santa Cruz Biotechnology (Santa Cruz, TX, USA).

Techniques: Migration, Derivative Assay, Histone Deacetylase Assay

Journal: International Journal of Molecular Sciences

Article Title: Mechanism Actions of Coniferyl Alcohol in Improving Cardiac Dysfunction in Renovascular Hypertension Studied by Experimental Verification and Network Pharmacology

doi: 10.3390/ijms251810063

Figure Lengend Snippet: Information on core targets.

Article Snippet: Antibodies against cyclooxygenase 2 (COX2) (sc-514489) and matrix metallopeptidase 9 (MMP9) (sc-393859) were procured from Santa Cruz Biotechnology (Santa Cruz, TX, USA).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Mechanism Actions of Coniferyl Alcohol in Improving Cardiac Dysfunction in Renovascular Hypertension Studied by Experimental Verification and Network Pharmacology

doi: 10.3390/ijms251810063

Figure Lengend Snippet: Molecular docking of CA with the core targets. Molecular docking of CA with the top six intersection targets. CA interacts with MMP9 via GLU-402, MET-422, and ARG-424 ( A ). CA interacts with COX2 via ALA-199, and ASN-382 ( B ). CA interacts with TNF α via GLU-94, GLU-95, and LEU-97 ( C ). CA interacts with IL-17 via GLU-94, GLU-95, and LEU-97 ( D ). CA interacts with TLR4 via GLU-225, ARG-227, PRO-202, and LEU-204 ( E ). CA interacts with NOS3 via GLU-272, ARG-255, and GLY-282 ( F ). CA and JUN have no interaction ( G ).

Article Snippet: Antibodies against cyclooxygenase 2 (COX2) (sc-514489) and matrix metallopeptidase 9 (MMP9) (sc-393859) were procured from Santa Cruz Biotechnology (Santa Cruz, TX, USA).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Mechanism Actions of Coniferyl Alcohol in Improving Cardiac Dysfunction in Renovascular Hypertension Studied by Experimental Verification and Network Pharmacology

doi: 10.3390/ijms251810063

Figure Lengend Snippet: CA inhibited the expressions of TNF and IL-17 signaling pathway proteins. The serum levels of TNF α ( A ) and IL-17 ( B ) were measured using commercially available ELLSA kits after the administration of 10 mg/kg of BENA, 20 mg/kg of CA, and 40 mg/kg of CA, respectively, to the RH mice. The expressions of TNF α ( C ), IL-17 ( D ), COX2 ( E ), and MMP9 ( F ) were detected by Western blot analysis after the administration of 10 mg/kg of BENA, 20 mg/kg of CA, and 40 mg/kg of CA, respectively, to the RH mice. The quantification of normalized TNF α, IL-17, COX2, and MMP9 was performed. In the quantitative chart, green circles represent sham group, pink squares represent model group, orange triangles represent 10 mg/kg BENA group, purple rhombuses represent 20 mg/kg CA group, and blue hexagons represent 40 mg/kg CA group. The values are mean ± SD, n = 5. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. sham; # p < 0.05, ## p < 0.01, and ### p < 0.001 vs. model.

Article Snippet: Antibodies against cyclooxygenase 2 (COX2) (sc-514489) and matrix metallopeptidase 9 (MMP9) (sc-393859) were procured from Santa Cruz Biotechnology (Santa Cruz, TX, USA).

Techniques: Western Blot

Journal: International Journal of Molecular Sciences

Article Title: Mechanism Actions of Coniferyl Alcohol in Improving Cardiac Dysfunction in Renovascular Hypertension Studied by Experimental Verification and Network Pharmacology

doi: 10.3390/ijms251810063

Figure Lengend Snippet: The inhibitory effect of CA on Ang II-stimulated hypertrophy of H9C2 cells. H9C2 cells were exposed to different concentrations of CA (0.5, 5, and 50 μM) under the stimulation of 5 μM Ang II. The expressions of α-SMA ( A ), TNF α ( B ), IL-17 ( C ), COX2 ( D ), and MMP9 ( E ) were detected by Western blot analysis. The quantification of normalized α-SMA, TNF α, IL-17, COX2, and MMP9 was performed. In the quantitative chart, green circles represent control, pink squares represent 5 μM Ang II, orange triangles represent 5 μM Ang II + 0.5 μM CA, purple rhombuses represent 5 μM Ang II + 5 μM CA, and blue hexagons represent 5 μM Ang II + 50 μM CA The values are mean ± SD, n = 5. * p < 0.05 vs. control; # p < 0.05, and ### p < 0.001 vs. 5 μM Ang II.

Article Snippet: Antibodies against cyclooxygenase 2 (COX2) (sc-514489) and matrix metallopeptidase 9 (MMP9) (sc-393859) were procured from Santa Cruz Biotechnology (Santa Cruz, TX, USA).

Techniques: Western Blot, Control